Difference between revisions of "Part:BBa K1061006"
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Hbax, a member of Bcl-2 family, is an apoptosis-related protein. It integrates into the mitochondrial outer membrane, releases cytochrome C and leads to apoptosis.Hbax184a is a mutant of hbax that constantly integrates into mitochondrial outer membrane. We have tried to use them as suicide genes, but they can not induce apoptosis in most cancer cell lines, probably because the pathway that they involve has been blocked in most cancer cells. However, we find that the protein can induce “apoptosis” in the most broadly used eukaryotic chassis, yeast. So it will be very potential to use the gene in some circuit that need to kill the yeast in proper condition. | Hbax, a member of Bcl-2 family, is an apoptosis-related protein. It integrates into the mitochondrial outer membrane, releases cytochrome C and leads to apoptosis.Hbax184a is a mutant of hbax that constantly integrates into mitochondrial outer membrane. We have tried to use them as suicide genes, but they can not induce apoptosis in most cancer cell lines, probably because the pathway that they involve has been blocked in most cancer cells. However, we find that the protein can induce “apoptosis” in the most broadly used eukaryotic chassis, yeast. So it will be very potential to use the gene in some circuit that need to kill the yeast in proper condition. | ||
====Calibration==== | ====Calibration==== | ||
| − | This is the pre-experment that we done. We inserted the hbax mutant form under the control of Gal promoter, than put it under the induction of the galatose. From left to right, the induction concentration is 0, 1%, and 2%. We can see a significantly decrease of yeast concentration when put it in a high level of induction(2%) | + | This is the pre-experment that we done. |
| + | We inserted the hbax mutant form under the control of Gal promoter, | ||
| + | than put it under the induction of the galatose. From left to right, the | ||
| + | induction concentration is 0, 1%, and 2%. We can see a significantly decrease | ||
| + | of yeast concentration when put it in a high level of induction(2%) | ||
[[Image:Hbax pre-experiment.png|thumb|center|800 × 536|pre-gradient experiment that we done.]] | [[Image:Hbax pre-experiment.png|thumb|center|800 × 536|pre-gradient experiment that we done.]] | ||
Revision as of 18:03, 23 September 2013
Media:Example.ogg Bax mutant form Hbax, a member of Bcl-2 family, is an apoptosis-related protein. It integrates into the mitochondrial outer membrane, releases cytochrome C and leads to apoptosis.Hbax184a is a mutant of hbax that constantly integrates into mitochondrial outer membrane. We have tried to use them as suicide genes, but they can not induce apoptosis in most cancer cell lines, probably because the pathway that they involve has been blocked in most cancer cells. However, we find that the protein can induce “apoptosis” in the most broadly used eukaryotic chassis, yeast. So it will be very potential to use the gene in some circuit that need to kill the yeast in proper condition.
Calibration
This is the pre-experment that we done.
We inserted the hbax mutant form under the control of Gal promoter,
than put it under the induction of the galatose. From left to right, the
induction concentration is 0, 1%, and 2%. We can see a significantly decrease
of yeast concentration when put it in a high level of induction(2%)
