Difference between revisions of "Part:BBa K1045015"

 
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<partinfo>BBa_K1045015 short</partinfo>
 
<partinfo>BBa_K1045015 short</partinfo>
  
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To be continued
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his part consists of BBa_K1045014 (a weak, inverted promoter upstream of a GFP transcription unit with a strong promoter and the DarR operator) with an inverse, strong RBS BBa_K1045010 upstream of the inverse promoter (BBa_K1045011). This part could be used to clone an inverse protein coding sequence upstream of the inverse RBS allowing it to be expressed from the weak promoter, while GFP is expressed from a strong promoter. In addition, GFP expression could be controlled by DarR (BBa_K1045001), if present.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:45, 23 September 2013

RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240

his part consists of BBa_K1045014 (a weak, inverted promoter upstream of a GFP transcription unit with a strong promoter and the DarR operator) with an inverse, strong RBS BBa_K1045010 upstream of the inverse promoter (BBa_K1045011). This part could be used to clone an inverse protein coding sequence upstream of the inverse RBS allowing it to be expressed from the weak promoter, while GFP is expressed from a strong promoter. In addition, GFP expression could be controlled by DarR (BBa_K1045001), if present.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
    Illegal NheI site found at 44
    Illegal NheI site found at 124
    Illegal NheI site found at 147
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 845