Difference between revisions of "Part:BBa K1065310"

Figure 1. Different light sources induction power We tested different light sources in order to define in which conditions our device is actually switched on and which condition effectively turns the transcription off. We grew a culture of transformed cells until it reached an OD = 0.7 (after , then we split the culture in 4 samples (5ml) and exposed them to condition:
dark control (the glass tube was wrapped with aluminum foil) (1); blue light bulb (2); white light (3); blue LED light (4);
After an induction period of about 8 hours, in which cultures grew at 37 degrees with stirring, we centrifuged cultures and observed that blue LED, blue bulb and white light all induced successfully the transcription of the chromoprotein, instead the dark control stayed uncoloured. White light worked as an activator, probably because it includes the right wavelength (470 nm). We could notice also that blue bulb illumination provoked a little less efficient induction.

Figure 2. Our experiment setup involving different types of light

Figure 3 and 4. Massive induction under illumination and prevented transcription at dark: liquid cultures and pellets. The images show the result of the test after an exposition time of 10 hours. As we can notice from the pellets, blue chromoprotein production occurred massively only in the blue LED exposed sample (1) and in the one exposed to regular light (2); dark definitely prevented amilCP to be produced (3). However from the liquid cultures we can infer that the blue LED worked more efficiently than the white light.

Figure 5. Absorbance spectra of induced samples and control: Since amilCP is a chromoprotein that absorbes in the UV/VIS range (peak at 588), we took some measurement at the spectrometer (PerkinElmer lambda 25) to have some quantitative data of the induction experiment. Data shown are relative to three samples induced for 9 hours. We considered a range between 400 nm and 700 nm. The graph shows that dark (green) significantly inhibited the device. It is also confirmed that white light (red) somehow induced a little less than the blue LE (blue).