Difference between revisions of "Part:BBa K1073001"
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<partinfo>BBa_K1073001 short</partinfo> | <partinfo>BBa_K1073001 short</partinfo> | ||
| − | This construct is derived from BBa_R0071 and | + | This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of ''P. aeruginosa'' which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS. The device can be cloned in front of a desired gene of interest in order to induce its Expression by N-buturyl-HSL. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The inducible Promotor from ''Pseudomonas aeruginosa'' is induced by RhlR complexed with the specific autoinducer butanoyl-homoserine lactone produced by RhlI. In ''Pseudomonas aeruginosa'' the induction of the promoter leads to activation of transcription of different virulence-associated traits in the cell [Medina 2003]. | ||
| + | For further Information see BBa_R0071. | ||
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Revision as of 07:38, 23 September 2013
Inducible promoter of the rhl quorum sensing system with RBS
This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of P. aeruginosa which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS. The device can be cloned in front of a desired gene of interest in order to induce its Expression by N-buturyl-HSL.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]