Difference between revisions of "Part:BBa K1210000"
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This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.  
 
This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.  
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<br><br>
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Datasheet for Part BBa_K542010 in <i>E. coli</i> strain DH5α.
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<br>
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[[File:PK401_data_sheet.pdf]]
  
 
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Revision as of 03:06, 23 September 2013

LacI-CFP-PK401-YFP

Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If translation continues in the 0 frame, a stop codon will be reached to give CFP as the final product. If frameshifting occurs due to the PK401 pseudoknot, translation will continue in the -1 frame to produce a CFP-YFP fusion protein with which FRET can be measured.

This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.

Datasheet for Part BBa_K542010 in E. coli strain DH5α.
File:PK401 data sheet.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]