Difference between revisions of "Part:BBa K1080010:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
Design Considerations here
+
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
 
+
 
+
  
 
===Source===
 
===Source===

Revision as of 01:53, 23 September 2013


YCF54


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 270


Design Notes

Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence

Source

Chlamydomonas reinhardtii

References