Difference between revisions of "Part:BBa K1080002:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence | ||
===Source=== | ===Source=== | ||
Revision as of 01:41, 23 September 2013
ChlD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1334
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2039
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 614
Illegal NgoMIV site found at 1433
Illegal NgoMIV site found at 1975 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
Source
Chlamydomonas reinhardtii