Difference between revisions of "Part:BBa K1188000"

 
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<partinfo>BBa_K1188000 short</partinfo>
 
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This part has  a strong constitutive promoter (J23100) the standard RBS (B0034) and AmilCP (K592009). When assembled into a plasmid it gives a strong blue color.  
 
This part has  a strong constitutive promoter (J23100) the standard RBS (B0034) and AmilCP (K592009). When assembled into a plasmid it gives a strong blue color.  
  
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The reason we created this part is the default backones all have essentially the same construct but using RFP instead. With an insert with a different color it made it much easier to debug problems with digests, ligations and transformations. We also used it to try to find a problem with some strange background issues we had.
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The reason we created this part is the default backones all have essentially the same construct but using RFP instead. Using an insert with a different color it made it much easier to debug problems with digests, ligations and transformations. We also used it to try to find a problem with some strange background issues we had.
  
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This was part also use to create a lot of AmilCP that was used in our protein purification experiments.
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This part is also very useful for protein purification experiments. In a standard 0.5% agarose gel it has an advantage of running up on the gel while RFP runs down on a gel. AmilCP absorbs at 588 nm while RFP excites at t 584 nm and emits at t 607 nm. However RFP has a secondary absorption peak at 502 nm and this is far enough from the 588 nm peak for AmilCP that it works extremely well. This allows AmilCP and RFP to be mixed and separated with an easy verification to figure out how effective the separation was.
  
 
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Revision as of 22:06, 22 September 2013

AmilCP screening part

This part has a strong constitutive promoter (J23100) the standard RBS (B0034) and AmilCP (K592009). When assembled into a plasmid it gives a strong blue color.

The reason we created this part is the default backones all have essentially the same construct but using RFP instead. Using an insert with a different color it made it much easier to debug problems with digests, ligations and transformations. We also used it to try to find a problem with some strange background issues we had.

This part is also very useful for protein purification experiments. In a standard 0.5% agarose gel it has an advantage of running up on the gel while RFP runs down on a gel. AmilCP absorbs at 588 nm while RFP excites at t 584 nm and emits at t 607 nm. However RFP has a secondary absorption peak at 502 nm and this is far enough from the 588 nm peak for AmilCP that it works extremely well. This allows AmilCP and RFP to be mixed and separated with an easy verification to figure out how effective the separation was.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]