Difference between revisions of "Help:Tag"
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| + | <small>Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Tag Tag parts]!<small> | ||
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Tag [Degradation] Tags] are genetic additions to the end of a sequence which mark a protein for degradation by various proteases within the cell. This effectively decreases the protein's half life<br> | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Tag [Degradation] Tags] are genetic additions to the end of a sequence which mark a protein for degradation by various proteases within the cell. This effectively decreases the protein's half life<br> | ||
One of the useful aspects of genetic tags is the ability to detect gene activity in a time-sensitive manner. | One of the useful aspects of genetic tags is the ability to detect gene activity in a time-sensitive manner. | ||
Revision as of 21:33, 13 July 2006
Browse Tag parts!
[Degradation Tags] are genetic additions to the end of a sequence which mark a protein for degradation by various proteases within the cell. This effectively decreases the protein's half life
One of the useful aspects of genetic tags is the ability to detect gene activity in a time-sensitive manner.
Tags are often found on Reporter parts and other types of Protein Coding parts.
"LVA, AAV, ASV, LAA" Tags
These are amino acid sequences which can be added to the C-terminal end of a protein. These sequences mark the protein for immediate degradation by intracellular proteases which specifically target the C-terminus tail of proteins.
references:
- Keiler, KC et al. "Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA." 1996. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8584937 Pubmed]
- Andersen, JB et al. "New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria". 1998. [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9603842 link]
Future Plans
Our future plans for tags involve building proteins using biobricks. To read more on this project, visit Ira Phillip's work on biobrick modification.