Difference between revisions of "Help:Tag"

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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Tag [Degradation] Tags] are genetic additions to the end of a sequence which mark a protein for degradation by various proteases within the cell.  This effectively decreases the protein's half life<br>
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One of the useful aspects of genetic tags is the ability to detect gene activity in a time-sensitive manner.
  
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[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Tag Tags] are small functional sequences of amino acids.  Thus far, our functional tags fall into the categories of transport out of  cell, and degradation tags (ie. LVA tags) <br>
 
 
Tags are often found on [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=reporter Reporter parts] and other types of [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Coding Protein Coding parts].<br>
 
Tags are often found on [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=reporter Reporter parts] and other types of [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Coding Protein Coding parts].<br>
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Our future plans for tags involve building proteins using biobricks.  To read more on this project, visit [https://dspace.mit.edu/handle/1721.1/32535 Ira Phillip's work on biobrick modification].
 
  
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==LVA Tag==
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=="LVA, AAV, ASV, LAA" Tags==
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Leucine+Valine+Alanine is an amino acid sequence which can be added to the end of a protein.  This sequence marks the protein for immediate degradation by the cell.  <br>
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These are amino acid sequences which can be added to the C-terminal end of a protein.  These sequences mark the protein for immediate degradation by intracellular proteases which specifically target the C-terminus tail of proteins.   
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references: [[http://www.sciencemag.org/cgi/content/summary/271/5251/885h?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=keiler+LVA&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT Keiler et al]]<br>
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<br>
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references: <br>
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# Keiler, KC et al.  "Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA."  1996. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8584937 Pubmed]
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# Andersen, JB et al.  "New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria".  1998.  [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9603842 link]
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==Future Plans==
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Our future plans for tags involve building proteins using biobricks.  To read more on this project, visit [https://dspace.mit.edu/handle/1721.1/32535 Ira Phillip's work on biobrick modification].

Revision as of 21:28, 13 July 2006

[Degradation Tags] are genetic additions to the end of a sequence which mark a protein for degradation by various proteases within the cell. This effectively decreases the protein's half life
One of the useful aspects of genetic tags is the ability to detect gene activity in a time-sensitive manner.

Tags are often found on Reporter parts and other types of Protein Coding parts.

"LVA, AAV, ASV, LAA" Tags

These are amino acid sequences which can be added to the C-terminal end of a protein. These sequences mark the protein for immediate degradation by intracellular proteases which specifically target the C-terminus tail of proteins.

references:

  1. Keiler, KC et al. "Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA." 1996. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8584937 Pubmed]
  2. Andersen, JB et al. "New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria". 1998. [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9603842 link]

Future Plans

Our future plans for tags involve building proteins using biobricks. To read more on this project, visit Ira Phillip's work on biobrick modification.