Difference between revisions of "Part:BBa K1017402"

Line 5: Line 5:
 
The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-2 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.
 
The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-2 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.
  
 +
[[File:NCTU_sRNA-synthesis.png]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:28, 22 September 2013

sRNA+rRBS-2


The DNA sequence produces sRNA which can combine with the sequence of its target region on the rRBS-2 designed by us, blocking the gene expressions downstream. It is the temporary design which can be simply amplified.

NCTU sRNA-synthesis.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 82
    Illegal NotI site found at 88
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 97
    Illegal SpeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]