Difference between revisions of "Part:BBa K1017602"

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	<br>From chart 1., we can clearly found that the time to reach same O.D. value(0.8) various as the temperature changes.		<br>From chart 1., we can clearly found that the time to reach same O.D. value(0.8) various as the temperature changes.
−	[[File:NCTU_37RBS_result.png|center|600px|Chart 1.37°C RBS efficiency under different temperatures.]]	+	[[File:NCTU_37RBS_result.png|600px|center|Chart 1.37°C RBS efficiency under different temperatures.]]
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Revision as of 09:34, 22 September 2013

37℃ RBS + mGFP

This biobrick is comprised of 37 degrees Celsius RBS and mGFP. 37℃ RBS, a RBS which on/off is controlled by temperature. mRFP is a red fluorescent protein.
When the temperature is higher than 37℃, 37℃ RBS functions normally and the downstream gene can be expressed. In contrast with high temperature,37℃ RBS forms a hairpin structure that block the ribosome binding when lower 37℃. Thus, the downstream gene, mGFP, is not able to translate.

From chart 1., we can clearly found that the time to reach same O.D. value(0.8) various as the temperature changes.

Sequence and Features