Difference between revisions of "Part:BBa K1100011:Design"

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The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab.
 
The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab.
 
sfGFP from Ding Yu's Lab at Fudan University,
 
sfGFP from Ding Yu's Lab at Fudan University,
−
R0040 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, BOO15 from Kit.
+
R0040 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, B0015 from Kit.
  
 
===References===
 
===References===

Revision as of 12:27, 21 September 2013


B0015 terminator efficiency estimation device (with Csy4 system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164


Design Notes

In this device, we test the terminator efficiency in the consideration that the terminator is influenced by the downstream sequence.


Source

The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. sfGFP from Ding Yu's Lab at Fudan University, R0040 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, B0015 from Kit.

References