Difference between revisions of "Part:BBa K1154002"

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<partinfo>BBa_K1154002 short</partinfo>
 
<partinfo>BBa_K1154002 short</partinfo>
  
The Saccharomyces cerevisiae HIS3 gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic his3 mutation (e.g., his3-&#916;1). It also compliments Escherichia coli hisB mutations (e.g., &#916;hisB720::kan).
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The ''Saccharomyces cerevisiae HIS3'' gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic ''his3'' mutation (''e.g.'', ''his3-&#916;1''). It also compliments ''Escherichia coli hisB'' mutations (''e.g.'', ''&#916;hisB720::kan'').
  
 
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Latest revision as of 04:45, 21 September 2013

Saccharomyces cerevisiae imidazoleglycerol-phosphate dehydratase (IGPD)

The Saccharomyces cerevisiae HIS3 gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic his3 mutation (e.g., his3-Δ1). It also compliments Escherichia coli hisB mutations (e.g., ΔhisB720::kan).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 562
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]