Difference between revisions of "Part:BBa K1210000"
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<partinfo>BBa_K1210000 short</partinfo> | <partinfo>BBa_K1210000 short</partinfo> | ||
− | Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If | + | Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If translation continues in the 0 frame, a stop codon will be reached to give CFP as the final product. If frameshifting occurs due to the PK401 pseudoknot, translation will continue in the -1 frame to produce a CFP-YFP fusion protein with which FRET can be measured. |
This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios. | This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios. |
Revision as of 15:35, 20 September 2013
LacI-CFP-PK401-YFP
Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If translation continues in the 0 frame, a stop codon will be reached to give CFP as the final product. If frameshifting occurs due to the PK401 pseudoknot, translation will continue in the -1 frame to produce a CFP-YFP fusion protein with which FRET can be measured.
This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]