Difference between revisions of "Part:BBa K1017726"
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The following is the change pathway. | The following is the change pathway. | ||
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Revision as of 13:22, 20 September 2013
Pcons+B0030+pcya+B0032+ho1+B0030
Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part and we choose J23101. B0030 and B0032 are strong ribosome binding sites(RBS), but they have different strength. pcyA and hol1 are two requisite genes which are required for the biosynthesis to change heme into PCB. hol1 will oxidizes the heme group then generate biliverdin IXalpha, and pcyA converts biliverdin IXalpha into phycocyanobilin(PCB).
The following is the change pathway. [[1]]
![[1]](https://static.igem.org/mediawiki/2013/9/9b/Nctu_formosa_PCBsynthe.jpg%7Ccenter%7C300px){kind=link}
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 833
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 386
- 1000COMPATIBLE WITH RFC[1000]