Difference between revisions of "Part:BBa K1041002"

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[[image:BIORAD 2013-09-18 16hr 02min 2.JPG|thumb|left|Fig 1:Lane 1 contains and Bba_K1041002 cut with XbaI and NdeI and lane 2 uncut Bba_K1041002.]]
 
[[image:BIORAD 2013-09-18 16hr 02min 2.JPG|thumb|left|Fig 1:Lane 1 contains and Bba_K1041002 cut with XbaI and NdeI and lane 2 uncut Bba_K1041002.]]
  
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Revision as of 11:40, 20 September 2013

AntG Promoter + RFP Coding Device

Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterisation

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Restriction Digest

Part Bba_K1041002 was digested with enzymes XbaI and NdeI and compared to uncut DNA Fig 1.

Fig 1:Lane 1 contains and Bba_K1041002 cut with XbaI and NdeI and lane 2 uncut Bba_K1041002.



















Sequencing

The biobrick was sent off to a company for sequencing and the data recieved showed the DNA is good quality Fig 2,3,4..

Fig 2:K1041002 sequencing data part 1
Fig 3:K1041002 sequencing data part 2
Fig 4:K1041002 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence Fig 5,6