Difference between revisions of "Part:BBa K1017101"

Revision as of 10:46, 20 September 2013

Pred ( PompC + B0032 + LacI + J61048 + Plac)

The chimera Cph8 is consist of the phytochrome Cph1 and the histidine kinase domain and response-regulator from EnvZ-OmpR. Cph8 autophosphorlates ompR, then phosphorylated-ompR activates the ompC promoter and turn on the downstream gene. Red light inhibits the autophosphorylation of Cph8 and turns off the gene expression.

But we need to a biobrick can turn on when exposing to red light. So we add LacI and lac promoter to downstream genes.

Pred, including PompC,RBS(B0032), LacI, ter(J61048), and Plac, can be activated when exposed to red light. OmpR, the transcription factor of PompC, is active when being phosphorylated in dark and inactive under red light. When there is red light, the non phosphorylated OmpR cannot turn on PompC. Thus, Plac won't be repressed by the negative regulator, LacI. So the whole Pred can express genes downstream Plac. On the other hand, if there is no red light, the phosphorylated OmpR then turn on PompC, causing LacI to be expressed and repress Plac to translate the genes downstream.

Sequence and Features