Difference between revisions of "Part:BBa K1100006:Design"

 
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===Design Notes===
 
===Design Notes===
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Csy4 is a protein used to cleavage the mRNA so that the influence from UTR will be eliminated
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A optimized sfGFP with a Csy4 loci, can be used as a powerful reporter. You can get this part from BBa_K1100015 and other parts we have submitted.
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===Source===
 
===Source===
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Insulator: Csy4 loci via PCR,
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The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab.
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B0034 via PCR,
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sfGFP from Ding Yu's Lab at Fudan University
  
 
===References===
 
===References===
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Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.

Latest revision as of 10:19, 20 September 2013


optimized sfGFP with Csy4 insulator loci


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A optimized sfGFP with a Csy4 loci, can be used as a powerful reporter. You can get this part from BBa_K1100015 and other parts we have submitted.

Source

Insulator: Csy4 loci via PCR, B0034 via PCR, sfGFP from Ding Yu's Lab at Fudan University

References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.