Difference between revisions of "Part:BBa K1041002:Experience"
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===Sequencing=== | ===Sequencing=== | ||
| − | The biobrick was sent off to a company for sequencing. | + | The biobrick was sent off to a company for sequencing and the data we received back ''Fig''showed the DNA is good quality. |
| − | [[image: | + | [[image:K1041002 1.JPG|thumb|left|K1041002 sequencing data part 1]] |
| − | [[image: | + | [[image:K1041002 2.JPG|thumb|left|K1041002 sequencing data part 2]] |
| − | [[image: | + | [[image:K1041002 3.JPG|thumb|left|K1041002 sequencing data part 3]] |
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===BLAST Analysis=== | ===BLAST Analysis=== | ||
The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2'' | The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2'' | ||
Revision as of 08:51, 20 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Team NRP-UEA_Norwich 2013
Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.
Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
Sequencing
The biobrick was sent off to a company for sequencing and the data we received back Figshowed the DNA is good quality.
BLAST Analysis
The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2
User Reviews
UNIQ37805a58e75d8b75-partinfo-00000000-QINU UNIQ37805a58e75d8b75-partinfo-00000001-QINU