Difference between revisions of "Part:BBa K1076000:Design"

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===Design Notes===
 
===Design Notes===
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It was design taking in consideration we had to put in a conjugative plasmid (pNPTS138) in E. coli and further on trasform Shewanella putrefaciens by conjugation. To put into pNPTS138 we used primers with Apa1(5') and BamH1(3')ends. After in order to put into pSB1C3 we clonned it in Topo plasmids and cut with Xba1 and Spe1 to further ligation on pSB1C3-BBa_150032.
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It was design taking in consideration we had to put in a conjugative plasmid (pNPTS138) in E. coli and further on trasform Shewanella putrefaciens by conjugation. To put into pNPTS138 we used primers with Apa1(5') and BamH1(3')ends. After in order to put into pSB1C3 we clonned it in Topo plasmids and cut with Xba1 and Spe1 to further ligation on pSB1C3.
  
 
===Source===
 
===Source===

Revision as of 23:37, 19 September 2013


fatty acid metabolism regulator protein FadR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was design taking in consideration we had to put in a conjugative plasmid (pNPTS138) in E. coli and further on trasform Shewanella putrefaciens by conjugation. To put into pNPTS138 we used primers with Apa1(5') and BamH1(3')ends. After in order to put into pSB1C3 we clonned it in Topo plasmids and cut with Xba1 and Spe1 to further ligation on pSB1C3.

Source

It comes from amplified genomic sequence

References