Difference between revisions of "Part:BBa J3101:Design"
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===References=== | ===References=== | ||
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| + | Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly | ||
| + | [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8508775] | ||
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| + | [[Media:Enhancer_Sequence_Paper.pdf| PERKINS-BALDING D, DIAS D, and GLASGOW A:Location, Degree, and Direction of DNA Bending Associated | ||
| + | with the Hin Recombinational Enhancer Sequence and | ||
| + | Fis-Enhancer Complex ]] | ||
Revision as of 16:17, 12 July 2006
Recombinational Enhancer (RE) for Hin/Hix inverting
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The base pair change of a T from a C occurred during cloning but is located in the spacer region between the fis binding sites; therefore, we believe that it will still work.
The insertion is right before the biobrick ends and several bases after the distal fis binding site; therefore, we believe it will function correctly.
We chose this RE to use because after cloning it retained functional full biobricks and the mutations do not seem harmful to function. The other RE that were cloned retained the proper RE sequence but lost critical cut sites in the biobricks.
RE is cloned in plasmid pSB1A2.
Source
References
Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8508775]