Difference between revisions of "Part:BBa K1041003"

 
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<partinfo>BBa_K1041003 short</partinfo>
 
<partinfo>BBa_K1041003 short</partinfo>
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Team NRP-UEA_Norwich 2013 created this part using biobricks [[BBa_K1041000]] and [[BBa_K1041002]]. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and ligated in front of the promoter of BBa_K1041000 to create a new biobrick.
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Bba_J04450 and Bba_K1041000
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<!-- Add more about the biology of this part here
 
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Revision as of 12:07, 19 September 2013

Neomycin Resistance Reporter Gene Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041002. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and ligated in front of the promoter of BBa_K1041000 to create a new biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 704
    Illegal SapI.rc site found at 914