Difference between revisions of "Part:BBa K1140003"

 
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<partinfo>BBa_K1140003 short</partinfo>
 
<partinfo>BBa_K1140003 short</partinfo>
  
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The LacI protein (with LVA tag) is regulated by a 37 degrees riboswitch. The part has a constitutive promoter.  
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A construction that has a gene coding for the LacI transcription factor, which also has a LVA degradation tag. The LacI gene is transcribed through a constitutive promoter and a thermolabile RBS with an optimal translation temperature of 37°C. Two termination sites were added at the 3’ end of the gene.
  
 
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Revision as of 01:19, 19 September 2013

pCons + 37 oC RNA thermometer + LacI (LVA)

A construction that has a gene coding for the LacI transcription factor, which also has a LVA degradation tag. The LacI gene is transcribed through a constitutive promoter and a thermolabile RBS with an optimal translation temperature of 37°C. Two termination sites were added at the 3’ end of the gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 44
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]