Difference between revisions of "Part:BBa K1140002"

 
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<partinfo>BBa_K1140002 short</partinfo>
 
<partinfo>BBa_K1140002 short</partinfo>
  
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A Green fluorescent protein (with LVA tag) that is regulated by a 32 degrees riboswitch. The part includes a the promoter "pLac".
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The gene is under the regulation of a pLac promoter and a thermolabile ribosome binding site (RBS) that should allow for translation only at temperatures above 32°C. At the 3’ end of the gene dubbed “GFP-LVA” are two consecutive transcription termination regions.  
  
 
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Revision as of 01:07, 19 September 2013

pLac + 32 oC RNA thermometer + GFP(LVA)

The gene is under the regulation of a pLac promoter and a thermolabile ribosome binding site (RBS) that should allow for translation only at temperatures above 32°C. At the 3’ end of the gene dubbed “GFP-LVA” are two consecutive transcription termination regions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 797