Difference between revisions of "Part:BBa K1017726:Design"

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__NOTOC__
 
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<partinfo>BBa_K1017726 short</partinfo>
 
<partinfo>BBa_K1017726 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
we want to change heme into PCB
+
This part is which we use to change heme into phycocyanobilin(PCB). We use the two different RBS to prevent Homologous recombination, which will cause the mutation of this part.
  
 
===Source===
 
===Source===
  
−
assemble parts form iGEM distribution
+
assemble parts form iGEM distribution I115008, I115009, I115010
  
 
===References===
 
===References===
 +
Anselm Levskaya, Aaron A. Chevalier, Jeffrey J. Tabor, Zachary Booth Simpson, Laura A. Lavery, Matthew Levy, Eric A. Davidson, Alexander Scouras, Andrew D. Ellington, Edward M. Marcotte, Christopher A. Voigt.(2005). Engineering Escherichia coli to see light, Nature,438:441
 +
 +
Elva J. H. Robinson, Duncan E. Jackson,Mike Holcombe, Francis L. W. Ratnieks.(2005). ‘No entry’ signal in ant foraging, Nature,438:442

Latest revision as of 13:07, 18 September 2013

Pcons+B0030+pcya+B0032+ho1+B0030


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 833
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is which we use to change heme into phycocyanobilin(PCB). We use the two different RBS to prevent Homologous recombination, which will cause the mutation of this part.

Source

assemble parts form iGEM distribution I115008, I115009, I115010

References

Anselm Levskaya, Aaron A. Chevalier, Jeffrey J. Tabor, Zachary Booth Simpson, Laura A. Lavery, Matthew Levy, Eric A. Davidson, Alexander Scouras, Andrew D. Ellington, Edward M. Marcotte, Christopher A. Voigt.(2005). Engineering Escherichia coli to see light, Nature,438:441

Elva J. H. Robinson, Duncan E. Jackson,Mike Holcombe, Francis L. W. Ratnieks.(2005). ‘No entry’ signal in ant foraging, Nature,438:442