Difference between revisions of "Part:BBa K1114703"

Revision as of 23:35, 17 September 2013

BioBrick adaptation of MoClo Level 1 RFP Reporter

This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses RFP. It contains the Level 0 parts J23104_AB, BCD12_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 694
Illegal AgeI site found at 806
1000
COMPATIBLE WITH RFC[1000]

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-This is a BioBrick adaptation of a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114109:Design">BCD13_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
+This is a BioBrick adaptation of a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114109:Design">BCD12_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="https://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
 The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.