Difference between revisions of "Part:BBa K1041002:Experience"

Revision as of 11:21, 17 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Team NRP-UEA_Norwich 2013

Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1

K1041000 sequencing data part 2

K1041000 sequencing data part 3

BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2

User Reviews

UNIQ086fdb527ce7c871-partinfo-00000000-QINU UNIQ086fdb527ce7c871-partinfo-00000001-QINU

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 Team NRP-UEA_Norwich 2013 created this part using biobricks [[BBa_K1041000]] and [[BBa_K1041001]]. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.
-Characterisation of this biobrick involved comparisons with the original Bba_J04450 biobrick by performing transformations, restriction digests and BLAST analysis. We also had our biobrick sequenced.
+Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
 ===Sequencing===