Difference between revisions of "Part:BBa K1041002:Experience"

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===Team NRP-UEA_Norwich 2013===
 
===Team NRP-UEA_Norwich 2013===
 
Team NRP-UEA_Norwich 2013 created this part using biobricks [[BBa_K1041000]] and [[BBa_K1041001]]. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.
 
Team NRP-UEA_Norwich 2013 created this part using biobricks [[BBa_K1041000]] and [[BBa_K1041001]]. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.
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Characterisation of this biobrick involved comparisons with the original Bba_J04450 biobrick by performing transformations, restriction digests and BLAST analysis. We also had our biobrick sequenced.
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===Sequencing===
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The biobrick was sent off to a company for sequencing.
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[[image:3a vf2 0-540.JPG|thumb|left|K1041000 sequencing data part 1]]
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[[image:3a vf2 440-860.JPG|thumb|left|K1041000 sequencing data part 2 ]]
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[[image:3a vf2 860-.JPG|thumb|left|K1041000 sequencing data part 3]]
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===BLAST Analysis===
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The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2''
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===User Reviews===
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<!-- DON'T DELETE --><partinfo>BBa_K1041000 StartReviews</partinfo>
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<!-- Template for a user review
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{|width='80%' style='border:1px solid gray'
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|-
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|width='10%'|
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<partinfo>BBa_K1041000 AddReview number</partinfo>
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<I>Username</I>
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|width='60%' valign='top'|
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Enter the review inofrmation here.
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|};
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===User Reviews===
 
===User Reviews===

Revision as of 11:19, 17 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Team NRP-UEA_Norwich 2013

Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.

Characterisation of this biobrick involved comparisons with the original Bba_J04450 biobrick by performing transformations, restriction digests and BLAST analysis. We also had our biobrick sequenced.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2

User Reviews

UNIQabe57ad8c3e7c601-partinfo-00000000-QINU UNIQabe57ad8c3e7c601-partinfo-00000001-QINU

User Reviews

UNIQabe57ad8c3e7c601-partinfo-00000002-QINU UNIQabe57ad8c3e7c601-partinfo-00000003-QINU