Difference between revisions of "Part:BBa K1041001:Experience"

 
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K1041001===
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===Team NRP-UEA_Norwich 2013===
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Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of [[BBa_J04450]] using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part [[Bba_K1041002]].
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Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.
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===Sequencing===
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The biobrick was sent off to a company for sequencing.
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[[image:3a vf2 0-540.JPG|thumb|left|K1041000 sequencing data part 1]]
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[[image:3a vf2 440-860.JPG|thumb|left|K1041000 sequencing data part 2 ]]
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[[image:3a vf2 860-.JPG|thumb|left|K1041000 sequencing data part 3]]
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===BLAST Analysis===
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The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence ''fig.2''
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===User Reviews===
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<!-- Template for a user review
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{|width='80%' style='border:1px solid gray'
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|-
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<partinfo>BBa_K1041000 AddReview number</partinfo>
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<I>Username</I>
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Enter the review inofrmation here.
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|};
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===User Reviews===
 
===User Reviews===

Revision as of 11:08, 17 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Team NRP-UEA_Norwich 2013

Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of BBa_J04450 using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene and provide restriction sites for further cloning such as part Bba_K1041002.

Characterisation of this biobrick involved sequencing, restriction digests and BLAST analysis.

Sequencing

The biobrick was sent off to a company for sequencing.

K1041000 sequencing data part 1
K1041000 sequencing data part 2
K1041000 sequencing data part 3










BLAST Analysis

The data we recieved back from the sequencing company was aligned using BLAST with the expected DNA sequence fig.2

User Reviews

UNIQ9b805a16c9e8a6db-partinfo-00000000-QINU UNIQ9b805a16c9e8a6db-partinfo-00000001-QINU

User Reviews

UNIQ9b805a16c9e8a6db-partinfo-00000002-QINU UNIQ9b805a16c9e8a6db-partinfo-00000003-QINU