Difference between revisions of "Part:BBa K1039000"

Revision as of 09:59, 17 September 2013

attP site for phiC31 integrase

The attP site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).

Reference: Amy C. Groth, Michele P. Calos, Phage Integrases: Biology and Applications, Journal of Molecular Biology, Volume 335, Issue 3, 16 January 2004, Pages 667-678, ISSN 0022-2836, http://dx.doi.org/10.1016/j.jmb.2003.09.082. Sequence and Features Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 The attP site is the phage attachment site for the phiC31 phage. The site is recognized by phiC31 integrase. The phiC31 integrase catalyzes a unidirectional strand exchange between a 39 bp attP site and a 34 bp attB site (Groth et al., 2000). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).
+Reference: Amy C. Groth, Michele P. Calos, Phage Integrases: Biology and Applications, Journal of Molecular Biology, Volume 335, Issue 3, 16 January 2004, Pages 667-678, ISSN 0022-2836, http://dx.doi.org/10.1016/j.jmb.2003.09.082. Sequence and Features
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 ===Usage and Biology===