Difference between revisions of "Featured Parts:Conjugation parts"
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[https://parts.igem.org/cgi/partsdb/view.cgi?part_name=BBa_J01000 BBa_J01000]: OriTf - Origin of Transfer for the F-type conjugative plasmid  
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[https://parts.igem.org/cgi/partsdb/view.cgi?part_name=BBa_J01002 BBa_J01002] "OriTf" - Origin of Transfer for the F-type conjugative plasmid  
 
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[https://parts.igem.org/cgi/partsdb/view.cgi?part_name=BBa_J01001 BBa_J01001]
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[https://parts.igem.org/cgi/partsdb/view.cgi?part_name=BBa_J01003 BBa_J01003] "OriTr" - Origin of Transfer for the R-type conjugative plasmid
 
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[https://parts.igem.org/cgi/partsdb/view.cgi?part_name=BBa_J01002 BBa_J01002]  
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[https://parts.igem.org/cgi/partsdb/view.cgi?part_name=BBa_J01000 BBa_J01000] "TraJf" -
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[https://parts.igem.org/cgi/partsdb/view.cgi?part_name=BBa_J01001 BBa_J01001] "TraJr" -
 
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Revision as of 00:01, 31 March 2006

BBa_J01002 "OriTf" - Origin of Transfer for the F-type conjugative plasmid
BBa_J01003 "OriTr" - Origin of Transfer for the R-type conjugative plasmid
BBa_J01000 "TraJf" -
BBa_J01001 "TraJr" -

Background/About Conjugation:
Conj.jpg

Bacterial conjugation is the transfer of genetic material between cells. This behaviour is characterized by the presence of a specialized plasmid (small, circular piece of transferrable DNA most often found within bacteria) known as a "conjugative plasmid" .
The conjugative plasmid holds a distinct and specialized set of coding regions including the following

  • OriT (Origin of Transfer): Unlike other plasmids, the conjugative plasmid has its own origin of transfer.
  • TraJ (Transfer genes): The activation of this gene sets off a cascade of other plasmid genes (and thus corresponding protein expressions) which act in concert to form "mating" characteristics in the host bacterial cell. Such salient characteristics include:
    • the growth of a pilus (a long whip-like apparatus used for genetic transfer between cells)
    • formation of "surface exclusion proteins" which prevents the cell containing the conjugative plasmid from mating with other conjugative plasmids of its type

More Information/References:

  1. Frost, Ipp en-Ihler, Skurray. Microbial Rev 1994. Analysis and Sequence of the F Plasmid [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7915817&query_hl=2&itool=pubmed_docsum Pubmed]
  2. Wikipedia. "Bacterial Conjugation" [http://en.wikipedia.org/wiki/Bacterial_conjugation Link]
  3. Berkeley iGEM 2005 [http://parts.mit.edu/wiki/index.php/Berkeley_2005 Project]