Difference between revisions of "Part:BBa K1116001"

Latest revision as of 05:18, 17 September 2013

LacI with miR-FF5 and miR-FF3 target

This part is a LacI coding sequence with 4 miR-FF5 and 4 miR-FF3 target sites so it can be regulated with micro-RNA FF5 and FF3.It was designed as a intermediate product to construct BBa_K1116003 in our project. .We use this part to construct K1116003.However,K1116003 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have tested K1116003 .The results can be seen in K1116002's experience. [[1]] So the plasmid K1116001 works because K1116003 have been tested.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1120
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1116001 short</partinfo>
-It was designed as a intermediate product to construct BBa_K1116003  in our project.
+This part is a LacI coding sequence with 4 miR-FF5 and 4 miR-FF3 target sites so it can be regulated with micro-RNA FF5 and FF3.It was designed as a intermediate product to construct BBa_K1116003  in our project. .We use this part to construct K1116003.However,K1116003 cannot be assembled in pSB1C3 because target gene has ECOR1 restriction site .We have tested K1116003 .The results can be seen in K1116002's experience.  [[https://parts.igem.org/Part:BBa_K1116002:Experience]] So the plasmid K1116001 works because K1116003 have been tested.
 <!-- Add more about the biology of this part here