Difference between revisions of "Part:BBa K1216003"
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<partinfo>BBa_K1216003 short</partinfo> | <partinfo>BBa_K1216003 short</partinfo> | ||
| − | ''nagZ'' encodes β-N-Acetylglucosaminidase, a cytoplasmatic hydrolase which is involved in the murein tripeptide recycling pathway of ''E.coli''.[[File:β-N-Acetylglucosaminidase_3D.jpg|thumb| 3D representation of the β-N-Acetylglucosaminidase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4GVG RCSB] ]] | + | ''nagZ'' encodes β-N-Acetylglucosaminidase, a cytoplasmatic hydrolase which is involved in the murein tripeptide recycling pathway of ''E.coli''[[Part:BBa_K1216003#References|<sup>[1]</sup>]].[[File:β-N-Acetylglucosaminidase_3D.jpg|thumb| 3D representation of the β-N-Acetylglucosaminidase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=4GVG RCSB] ]] |
<!-- Add more about the biology of this part here--> | <!-- Add more about the biology of this part here--> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | β-N-Acetylglucosaminidase can be used as a reporter enzyme in a histochemical reaction with substrates like 5-Bromo-4-Chloro-3-Indolyl-N-Acetyl-β-D-Glucosaminide, which will after hydrolysis release a blue chromophore[[Part: | + | β-N-Acetylglucosaminidase can be used as a reporter enzyme in a histochemical reaction with substrates like 5-Bromo-4-Chloro-3-Indolyl-N-Acetyl-β-D-Glucosaminide, which will after hydrolysis release a blue chromophore[[Part:BBa_K1216003#References|<sup>[2]</sup>]]. |
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===References=== | ===References=== | ||
| + | # Cheng et al. (2000). Molecular Characterization of the b-N-Acetylglucosaminidase of Escherichia coli and Its Role in Cell Wall Recycling. ''Journal of Bacteriology''. 182: 4836-4840. | ||
# [http://www.inalcopharm.com/cart.php?m=product_detail&p=6 Inalcopharm datasheet] | # [http://www.inalcopharm.com/cart.php?m=product_detail&p=6 Inalcopharm datasheet] | ||
Revision as of 17:30, 16 September 2013
beta-N-Acetylglucosaminidase (nagZ) from Escherichia Coli
nagZ encodes β-N-Acetylglucosaminidase, a cytoplasmatic hydrolase which is involved in the murein tripeptide recycling pathway of E.coli[1].
Usage and Biology
β-N-Acetylglucosaminidase can be used as a reporter enzyme in a histochemical reaction with substrates like 5-Bromo-4-Chloro-3-Indolyl-N-Acetyl-β-D-Glucosaminide, which will after hydrolysis release a blue chromophore[2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 25
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 76
References
- Cheng et al. (2000). Molecular Characterization of the b-N-Acetylglucosaminidase of Escherichia coli and Its Role in Cell Wall Recycling. Journal of Bacteriology. 182: 4836-4840.
- [http://www.inalcopharm.com/cart.php?m=product_detail&p=6 Inalcopharm datasheet]