Difference between revisions of "Part:BBa K1164004"

Revision as of 07:01, 16 September 2013

pTRELX: Modified Gal1 promoter; gal4O replaced w/ tetO; repressable via lacO

This is a modified version of the native Gal1 promoter found in S.cerevisiae. The four gal4 binding domains present in the native promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites. In addition, two lacO operators have been introduced downstream of the TATA box, allowing for repression gene expression by lacI.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 465
Illegal BamHI site found at 553
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 81
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1164004 short</partinfo>
-This is a modified version of the native Gal1 promoter found in S.cerevisiae. The four gal4 binding domains present in the native promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites.
+This is a modified version of the native Gal1 promoter found in S.cerevisiae. The four gal4 binding domains present in the native promoter have been replaced by 4 tetO sites. This promoter may be activated by regulatory activators that are able to bind to the tetO sites. In addition, two lacO operators have been introduced downstream of the TATA box, allowing for repression gene expression by lacI.
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