Difference between revisions of "Part:BBa K1149006"

Revision as of 19:17, 15 September 2013

pelB-pulA-flag expression

Expression of a plastic degradation enzyme from Pseudomonas fluorescens under arabinose inducible promoter. The protein in codon optimized for expression in ecoli, is flag tagged and has an Nterminal pelB tag for secretion. Encodes polyurethane esterase, break down ester bonds in polyurethane.

PUR degradation by esterase:

references:

[http://www.sciencedirect.com/science/article/pii/S0964830598000687 Cloning and expression in Escherichia coli of a polyurethane-degrading enzyme from Pseudomonas fluorescens]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 804
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1099
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 Encodes polyurethane esterase, break down ester bonds in polyurethane.
-Theoretical pathway of PUR degradation by esterase:
+PUR degradation by esterase:
-https://static.igem.org/mediawiki/igem.org/f/fd/PUR_esterase_theoretical_pathway.jpg
+https://static.igem.org/mediawiki/parts/3/31/PUR_esterase_theoretical_pathway_2.jpg
 <p>references: </p>
-<p>[http://www.selu.edu/acad_research/depts/biol/faculty/publications/pdf/2012/howard2012.pdf Howard, Gary 2012 pg:371 -3, Polyurethane Biodegradation, Environmental Science]</p>
 <p>[http://www.sciencedirect.com/science/article/pii/S0964830598000687 Cloning and expression in Escherichia coli of a polyurethane-degrading enzyme from Pseudomonas fluorescens]</p>