Difference between revisions of "Part:BBa K1149004"
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| − | PUR degradation enzyme from | + | PUR degradation enzyme from Pseudomonas chlororaphis. |
https://static.igem.org/mediawiki/parts/3/31/PUR_esterase_theoretical_pathway_2.jpg | https://static.igem.org/mediawiki/parts/3/31/PUR_esterase_theoretical_pathway_2.jpg | ||
Revision as of 19:15, 15 September 2013
pelB-pueB expression
PUR degradation enzyme from Pseudomonas chlororaphis.
references:
[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)]
[http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 99
Illegal BglII site found at 1213 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 407
Illegal AgeI site found at 752
Illegal AgeI site found at 1241
Illegal AgeI site found at 1517 - 1000COMPATIBLE WITH RFC[1000]