Difference between revisions of "Part:BBa K1149004"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1149004 short</partinfo>
 
<partinfo>BBa_K1149004 short</partinfo>
  
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PUR degradation
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PUR degradation enzyme from
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https://static.igem.org/mediawiki/parts/3/31/PUR_esterase_theoretical_pathway_2.jpg
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<p>references: </p>
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<p> [http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] <p/>
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<p> [http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli] </P>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:14, 15 September 2013

pelB-pueB expression

PUR degradation enzyme from

PUR_esterase_theoretical_pathway_2.jpg

references:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] <p/> <p> [http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BglII site found at 1213
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 407
    Illegal AgeI site found at 752
    Illegal AgeI site found at 1241
    Illegal AgeI site found at 1517
  • 1000
    COMPATIBLE WITH RFC[1000]