Difference between revisions of "Part:BBa K365002"

Revision as of 22:03, 26 October 2010 (view source) Georgio (Talk | contribs) ← Older edit		Latest revision as of 18:59, 15 September 2013 (view source) Earthnut (Talk | contribs)
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	<partinfo>BBa_K365002 short</partinfo>		<partinfo>BBa_K365002 short</partinfo>
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		+	'''''Note: This is a N-part RFC[25] Biobrick which means only protein fusions to the C-terminus of this Biobrick is possible. For a improved version of this Biobrick which also allow fusion to the N-terminus of PhyB (e.&nbsp;g. ''Strep''-tag II or His-tag), please see here: [https://parts.igem.org/Part:BBa_K801031 BBa_K801031]'''''
	This part consists in the first 908 amino-acids of Phytochrome B of A. thaliana. When bound to its natural chromophore Phycocyanobiline (PCB) it can reach its Pfr activated state and physically interact with different PIF.		This part consists in the first 908 amino-acids of Phytochrome B of A. thaliana. When bound to its natural chromophore Phycocyanobiline (PCB) it can reach its Pfr activated state and physically interact with different PIF.
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−	<img src="https://static.igem.org/mediawiki/2010/e/e6/ESBS-Strasbourg-PhyB900-black.png" width="250px" height="167px" border="0">	+	<img src="https://static.igem.org/mediawiki/2010/e/e6/ESBS-Strasbourg-PhyB900-black.png" width="250px" height="167px" border="0"><br>
	<a target="_blank" href="http://2010.igem.org/Team:ESBS-Strasbourg/Project/visual"><font>You can visit our wiki "visual description" page for more info</font></a>		<a target="_blank" href="http://2010.igem.org/Team:ESBS-Strasbourg/Project/visual"><font>You can visit our wiki "visual description" page for more info</font></a>
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Latest revision as of 18:59, 15 September 2013

Phytochrome B from A. thaliana (aa 1-908)

Note: This is a N-part RFC[25] Biobrick which means only protein fusions to the C-terminus of this Biobrick is possible. For a improved version of this Biobrick which also allow fusion to the N-terminus of PhyB (e. g. Strep-tag II or His-tag), please see here: BBa_K801031

This part consists in the first 908 amino-acids of Phytochrome B of A. thaliana. When bound to its natural chromophore Phycocyanobiline (PCB) it can reach its Pfr activated state and physically interact with different PIF.

Background:

Phytochromes characterised by a red/far-red photochromicity. Through red-light (650–670 nm) absorption the phytochrome undergoes a rapid conformational change from its ground state Pr to its active state Pfr. The structural change allows the binding of the PIF. This light-sensitive interaction has been mapped to the 650-residue amino-terminal photosensory core of PhyB (Khanna et al., 2004). The process is completely reversible through absorption in the near infra-red spectrum (705–740 nm).

The photoreceptor protein PhyB serves for the light-dependent activation of the system, therefore it will be fused to the N-teminal of the ClpX-trimer.


You can visit our wiki "visual description" page for more info

Sequence and Features

Conception:

In in-vivo applications it has been shown that the PIF-interaction with the PhyB photosensory core (residues 1–650) is irreversible in infrared light. Lim & Voigt (2009) demonstrated by assaying PIF6 (which has the strongest interactions of all previously reported PIF domains) against different variants of PhyB that the tandem C-terminal PAS domains (residues 1-908)of plant phytochromes are necessary to confer rapid photoreversibility under infrared light. The original sequence contains a SpeI restriction within the first 908 residues.