Difference between revisions of "Part:BBa K157004"
| Line 2: | Line 2: | ||
<partinfo>BBa_K157004 short</partinfo> | <partinfo>BBa_K157004 short</partinfo> | ||
| − | '''''Note: There is a improved (triple mutant version) of this Biobrick with higher binding affinity for Fluorescein: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1159003 BBa_K1159003]''''' | + | '''''Note: There is a improved (triple mutant version) of this Biobrick with higher binding affinity for Fluorescein; this mutations are also designed and introduced by Skerra lab: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1159003 BBa_K1159003]''''' |
Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from ''Pieris brassicae'', originally designed by Arne Skerra [see Ref. 1,2]. | Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from ''Pieris brassicae'', originally designed by Arne Skerra [see Ref. 1,2]. | ||
Revision as of 18:26, 15 September 2013
Fluoresceine-A-binding
Note: There is a improved (triple mutant version) of this Biobrick with higher binding affinity for Fluorescein; this mutations are also designed and introduced by Skerra lab: BBa_K1159003
Fluoresceine A -binding derivative of a Lipocalin (BBP, bilin binding protein from Pieris brassicae, originally designed by Arne Skerra [see Ref. 1,2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]