Difference between revisions of "Part:BBa K1149002"

Revision as of 17:22, 15 September 2013

pelB-EstCS2-his expression (GFP reporter)

This part expresses a plastic degradation enzyme and GFP under the control of an arabinose inducible promoter.

The enzyme is the estCS2 from BBa_K892012 previous biobrick by Washington 2012 team, that encodes carboxylesterase that breaks down ester bonds in polyurethane. We have added a pelB seretion tag to the enzyme so that it can be secreted extacellularly. We also added a 6xHistidine tag for Western blotting and protein purification purposes.

Theoretical pathway of PUR degradation by esterase enzymes:

references: [http://www.selu.edu/acad_research/depts/biol/faculty/publications/pdf/2012/howard2012.pdf Howard, Gary yr:2012 pg:371 -3, Polyurethane Biodegradation, Environmental Science [1431-6250]]

[http://www.microbialcellfactories.com/content/10/1/41 A novel family VII esterase with industrial potential from compost metagenomic library. Microbial Cell Factories 2011, 10:41 doi:10.1186/1475-2859-10-41]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal BamHI site found at 692
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 210
Illegal NgoMIV site found at 958
Illegal NgoMIV site found at 1192
Illegal AgeI site found at 2131
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1006

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1149002 short</partinfo>
+This part expresses a plastic degradation enzyme and GFP under the control of an arabinose inducible promoter.
+The enzyme is the estCS2 from [https://parts.igem.org/Part:BBa_K892012 BBa_K892012] previous biobrick by Washington 2012 team, that encodes carboxylesterase that breaks down ester bonds in polyurethane. We have added a [https://parts.igem.org/Part:BBa_K1149023 pelB] seretion tag to the enzyme so that it can be secreted extacellularly. We also added a 6xHistidine tag for Western blotting and protein purification purposes.
+Theoretical pathway of PUR degradation by esterase enzymes:
+https://static.igem.org/mediawiki/igem.org/f/fd/PUR_esterase_theoretical_pathway.jpg
+references:
+[http://www.selu.edu/acad_research/depts/biol/faculty/publications/pdf/2012/howard2012.pdf Howard, Gary yr:2012 pg:371 -3, Polyurethane Biodegradation, Environmental Science [1431-6250]]
+[http://www.microbialcellfactories.com/content/10/1/41 A novel family VII esterase with industrial potential from compost metagenomic library. Microbial Cell Factories 2011, 10:41 doi:10.1186/1475-2859-10-41]
 <!-- Add more about the biology of this part here