Difference between revisions of "Part:BBa C0060"

Revision as of 15:08, 30 June 2006

autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone

Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.

Usage and Biology

The aiiA gene has 750 nucleotides and encodes a protein of 250aa with a predicted molecular mass of 28,036Da and an isoelectric point of 4.7. The protein has no hydrophobic signal pepetide at the N-terminus and therefore it is believe that it is not secreted. This is supported by the observation that when aiiA is expressed in E.coli DH5alpha or Bacillus 240B1 cells no autoinducer inactivation is detected in the supernatants of these cultures. The AiiA protein has no significant overall homology with other known proteins, but based on the presence of two well conserved motifs it is believed that it is a metalloenzyme.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 99
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-The &lt;em&gt;aiiA &lt;/em&gt;gene has 750 nucleotides and encodes a protein of 250aa      with a predicted molecular mass of 28,036Da and an isoelectric point of 4.7.      The protein has no hydrophobic signal pepetide at the &lt;em&gt;N&lt;/em&gt;-terminus      and therefore it is believe that it is not secreted. This is supported by      the observation that when &lt;em&gt;aiiA &lt;/em&gt;is expressed in &lt;em&gt;E.coli&lt;/em&gt; DH5alpha      or &lt;em&gt;Bacillus&lt;/em&gt; 240B1 cells no autoinducer inactivation is detected in      the supernatants of these cultures. The AiiA protein has no significant overall      homology with other known proteins, but based on the presence of two well      conserved motifs it is believed that it is a metalloenzyme.
+The <em>aiiA</em> gene has 750 nucleotides and encodes a protein of 250aa      with a predicted molecular mass of 28,036Da and an isoelectric point of 4.7.      The protein has no hydrophobic signal pepetide at the <em>N</em>-terminus      and therefore it is believe that it is not secreted. This is supported by      the observation that when <em>aiiA </em> is expressed in <em>E.coli</em> DH5alpha      or <em>Bacillus</em> 240B1 cells no autoinducer inactivation is detected in      the supernatants of these cultures. The AiiA protein has no significant overall      homology with other known proteins, but based on the presence of two well      conserved motifs it is believed that it is a metalloenzyme.
 <span class='h3bb'>Sequence and Features</span>