Difference between revisions of "Part:BBa K1124000:Design"
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Revision as of 02:57, 12 September 2013
amilGFP (+LVA), yellow reporter protein with degradation tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LVA degradation tag was added to increase protein degradation. .
13 amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site, which may not be necessary to increase protein decay. LVA tag is the last 11 aa(sequence: AANDENYALVA) .1
Source
PCR (template: BBa_K592010, primers are shown on our wiki (UT-Tokyo 2013)(currently under construction). )
References
1 Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.