Difference between revisions of "Part:BBa K1072010"
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If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ. | If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ. | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/4/49/Odr-10.jpg" style="width:700px;margin-left:10%;"/> | ||
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| + | <p>Figure 1. Comperison between endogenous GPCR pathway in yeast and motified Odr10 pathway.</p> | ||
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Revision as of 08:36, 10 September 2013
Odr-10(diacetyl)GPCR with optimized codon
Odr-10(diacetyl)GPCR encodes a diacetyl-sensing G-protein coupled receptor, originating from the C. elegans.
If BBa_K1072010 was transformed into the yeast, the pheromone receptors will be actived and coupled to a heterotrimeric G protein, Odr-10. pathway is composedof Gpa1p (Gα), Ste4p (Gβ) and Ste18p (Gγ) subunits.The Gβγ particle transmits the signal to a MAP kinase module and finally follow a inducible promotor FUS1, which will links a reproter, such as GFP, His3 or LacZ.
Figure 1. Comperison between endogenous GPCR pathway in yeast and motified Odr10 pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]