Difference between revisions of "Part:BBa J45002:Design"

(→‎Design Notes)
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#Dudareva pmid=11051108
 
#Dudareva pmid=11051108
 
</biblio>
 
</biblio>
−
#Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
+
#Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA AGT GAT GAA GAA AC </b>
−
#Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC TAC
+
#Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA<b>T CTC CTA CTT AGA GAA ACT AC </b>
  
  

Revision as of 15:10, 26 June 2006


SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • There are two Met codons at the N-terminus of the protein (putative codons 1 and 4). It is believed that the first Met codon is the actual start of the protein. Reference:

<biblio>

  1. Dudareva pmid=11051108

</biblio>

  1. Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA AGT GAT GAA GAA AC
  2. Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAT CTC CTA CTT AGA GAA ACT AC


  • Bases 1-18 of the original A. majus sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon.
  • A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.
  • The original stop codon (TGA at bp 1093) was deleted and a double TAA stop was inserted

Source

Genbank accession number(s): AF198492

We got this cDNA from N Dudareva at Purdue.

References