Difference between revisions of "Part:BBa K1123012:Design"

 
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===Design Notes===
 
===Design Notes===
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No adaptations were made as the part has already been optimized for E.coli use.
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This part was left mainly unchanged as it was lifted directly from the pET28-a vector. However we did add a NheI restriction site to the end which we would be able to use to restrict the protein which we would be placing behind the His-Tag. These are the last 6 base pairs at the end of the sequence.
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===Source===
 
===Source===

Latest revision as of 12:12, 8 September 2013


His-Tag and Thrombine Cleavage Site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was left mainly unchanged as it was lifted directly from the pET28-a vector. However we did add a NheI restriction site to the end which we would be able to use to restrict the protein which we would be placing behind the His-Tag. These are the last 6 base pairs at the end of the sequence.

Source

The DNA sequence for this part was lifted directly from the pET28-a vector. This will allow us to use this part without further adaptations.

References