Difference between revisions of "Part:BBa K1114107:Design"

Revision as of 19:58, 7 September 2013

Bicistronic Design RBS 2, MoClo Format with BC fusion sites

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.

Source

This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= “http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html”?>Mutalik, et al.</a>

References

<a href= “http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html”?>Mutalik, et al.</a>

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 ===Design Notes===
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-This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. See the <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> page for full information on the part.
+This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites.
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 </html>
 ===Source===