Difference between revisions of "Featured Parts:Conjugation parts"
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* OriT (Origin of Transfer): Unlike other plasmids, the conjugative plasmid has its own origin of transfer. | * OriT (Origin of Transfer): Unlike other plasmids, the conjugative plasmid has its own origin of transfer. | ||
* TraJ (Transfer genes): The activation of this set of genes on the conjugative plasmid sets off a cascade of protein activation that forces the host cell to start expressing surface proteins that form "mating" characteristics. Such salient characteristics include: | * TraJ (Transfer genes): The activation of this set of genes on the conjugative plasmid sets off a cascade of protein activation that forces the host cell to start expressing surface proteins that form "mating" characteristics. Such salient characteristics include: | ||
| − | + | ** the growth of a pilus (a long whip-like apparatus used for genetic transfer between cells) | |
| − | + | ** formation of "surface exclusion proteins" which prevents the cell containing the conjugative plasmid from mating with other conjugative plasmids of its type (ie. F-type plasmid bearers manufacture surface exclusion proteins that prevent it from mating with other F-type cells although it will mate with cells bearing the R-type plasmid) | |
| − | References: | + | References:<br> |
| − | 1. Frost, | + | 1. Frost, Ipp en-Ihler, Skurray. Analysis and Sequence of the F Plasmid [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7915817&query_hl=2&itool=pubmed_docsum Pubmed]<br> |
2. Wikipedia. "Bacterial Conjugation" [http://en.wikipedia.org/wiki/Bacterial_conjugation Link] | 2. Wikipedia. "Bacterial Conjugation" [http://en.wikipedia.org/wiki/Bacterial_conjugation Link] | ||
</small> | </small> | ||
| + | 3. Berkeley iGEM 2005 Project [[Berkeley]] | ||
Revision as of 02:17, 30 March 2006
Part Type: Conjugation Parts
About Conjugation:
Bacterial conjugation is the transfer of genetic material between cells. This behaviour is characterized by the presence of a specialized plasmid (small, circular piece of transferrable DNA most often found within bacteria) known as a "conjugative plasmid" .
The conjugative plasmid holds on it a distinct and specialized set of coding regions including the following
- OriT (Origin of Transfer): Unlike other plasmids, the conjugative plasmid has its own origin of transfer.
- TraJ (Transfer genes): The activation of this set of genes on the conjugative plasmid sets off a cascade of protein activation that forces the host cell to start expressing surface proteins that form "mating" characteristics. Such salient characteristics include:
- the growth of a pilus (a long whip-like apparatus used for genetic transfer between cells)
- formation of "surface exclusion proteins" which prevents the cell containing the conjugative plasmid from mating with other conjugative plasmids of its type (ie. F-type plasmid bearers manufacture surface exclusion proteins that prevent it from mating with other F-type cells although it will mate with cells bearing the R-type plasmid)
References:
1. Frost, Ipp en-Ihler, Skurray. Analysis and Sequence of the F Plasmid [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7915817&query_hl=2&itool=pubmed_docsum Pubmed]
2. Wikipedia. "Bacterial Conjugation" [http://en.wikipedia.org/wiki/Bacterial_conjugation Link]
3. Berkeley iGEM 2005 Project Berkeley