Difference between revisions of "Part:BBa K1114033:Design"

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<partinfo>BBa_K1114033 short</partinfo>
 
<partinfo>BBa_K1114033 short</partinfo>
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<partinfo>BBa_K1114033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1114033 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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In a MoClo backbone, <a href="">BBa_DVL0_FB</a>.
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_R0079 with FB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_R0079">BBa_R0079</a> page for full information on the part.  
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This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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Summary of modifications from original part:
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_?????“>BBa_??????</a>.
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</html>
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===Source===
 
===Source===
  
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iGEM Kit
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iGEM Distribution Kit
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===References===
 
===References===

Latest revision as of 18:27, 7 September 2013

MoClo formatted part of BBa_R0079 with FB fusion sites



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of BBa_R0079 with FB fusion sites. See the BBa_R0079 page for full information on the part. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_??????.


Source

iGEM Distribution Kit


References