Difference between revisions of "Part:BBa K1114000:Design"

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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23100 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a> page for full information on the part.  
 
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23100 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a> page for full information on the part.  
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K1114102“>BBa_K783055/a>.
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href=”https://parts.igem.org/Part:BBa_K1114102“>BBa_K783055</a>.
  
  
 
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===Source===
 
===Source===

Latest revision as of 18:26, 7 September 2013


The MoClo format of BBa_J23100 with AB fusion sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of BBa_J23100 with AB fusion sites. See the BBa_J23100 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.

Source

iGEM Distribution Kit


References

Source

iGEM Distribution Kit

References